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Our Database/Tools
T2TCottonHub
A Comprehensive Database containing T2T-Jin668, GapLess-YZ1 and TM-1 genome resources used for Functional Genomics Research in Cotton.
T2T Cotton-Hub
CRISPRone
An integrated CRISPR community encompassing target sgRNA design, editing results detection, and large language models related to CRISPR.
CRISPR All in One
CLC
A comprehensive scRNA database that developed for providing the transcriptomic landscape of major cell types of cotton at single-cell resolution.
Cell Landscape Cotton
PsctH
A comprehensive and accurate resource of cell markers and web tool for various cell types in tissues of plant species to explore single-cell landscape.
Plant Single Cell HubLatest News
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Latest Publications
Single-cell resolution analysis reveals the preparation for reprogramming the fate of stem cell niche in cotton lateral meristem
- Date: 25 August, 2023
- Journal: Genome Biology
- Authors: Xiangqian Zhu, Zhongping Xu, Guanying Wang, Yulong Cong, Lu Yu, Ruoyu Jia, Yuan Qin, Guangyu Zhang, Bo Li, Daojun Yuan, Lili Tu, Xiyan Yang, Keith Lindsey, Xianlong Zhang & Shuangxia Jin
- Results: We generate a high-resolution single-cell transcriptomic landscape of hypocotyl tissue from the highly regenerable cotton genotype Jin668 and the recalcitrant TM-1. We identify nine putative cell clusters and 23 cluster-specific marker genes for both cultivars and find that the primary vascular cell is the major cell type that undergoes cell fate transition in response to external stimulation. Further developmental trajectory and gene regulatory network analysis of these cell clusters reveals that a total of 41 hormone response-related genes, including LAX2, LAX1, and LOX3, exhibit different expression patterns in the primary xylem and cambium region of Jin668 and TM-1. We also identify novel genes, including CSEF, PIS1, AFB2, ATHB2, PLC2, and PLT3, that are involved in regeneration.
- Keywords: Cotton, Plant regeneration, scRNA-seq, Gene regulatory network, Gene functional verification
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Latest Publications
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Our Contributions
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