Required:
1. What is the project name? Usually is gene id
2. The base primer of Forward from 5' to 3'
3. The base primer of Reverse from 5' to 3'
4. Barcode length; Enter LENGTH as an integer (i.e. 4)
5. Total number of barcodes (Primer Pairs) (default is LENGTH x 5)
Optional:
6. The minimum number of different bases between barcodes (default is LENGTH/2, i.e. 7->3, 4->2)
7. Desired GC content minimum range in percentages (i.e. 50 ->50%%) (default is 0)
8. Desired GC content maximum range in percentages (i.e. 50 ->50%%) (default is 100)
9. How many attempts? The default number of random codes to test is 10000.
10. Prepare for sent to company?