Adelphocoris suturalis Jakovlev
Adelphocoris suturalis (Hemiptera: Miridae) is a notorious agricultural pest, which causes serious economic losses to a diverse range of agricultural crops around the world. The poor understanding of its genomic characteristics has seriously hindered the establishment of sustainable and environment-friendly agricultural pest management through biotechnology and biological insecticides.
Here, we report a chromosome-level assembled genome of A. suturalis by integrating Illumina short reads, PacBio, 10x Chromium, and Hi-C mapping technologies. The 1.29 Gb assembly contains twelve chromosomal pseudomolecules with a contig and scaffolds N50 of 2.8 Mb and 120.6 Mb, respectively.
A comprehensive gene model prediction integrated with homology-based prediction, RNA-sequencing-assisted prediction and ab initio prediction identified a total of 20,010 protein-coding genes with 86.4% complete BUSCO genes. Of these, more than 94.3% genes are functionally annotated following searches of NR, GO, KEGG, Swiss-Prot and TrEMBL databases.
|Sequencing platform||Illumina+PacBio RS Ⅱ+PacBio Sequel Ⅱ|
|Genome sequencing depth (x)||260|
|Genome sequencing depth HiC||106|
|Estimated genome size (Gb)||1.29|
|Number of scaffolds||98|
|Sequenced genome size (Gb)||1.29|
|Contig N50 (Mb)||1.43|
|Scaffold N50 (Mb)||120.63|
|Longest scaffold (Mb)||188.72|
|GC content (%)||38.38|
|Repetitive sequences (%)||72.76|
|Annotated protein-coding genes||20,010|
|BUSCO completeness of assembly (%)||95.3|
|BUSCO completeness of annotation (%)||86.4|
Zhongping Xu, Guanying Wang, Jing Luo, Mingju Zhu, Lisong Hu, Sijia Liang, Bo Li, Xingxing Huang, Ying Wang, Guangyu Zhang, Can Zhang, Yi Zhou, Daojun Yuan, Taiyu Chen, Lizhen Chen, Weihua Ma, Wei Gao, Keith Lindsey, Xianlong Zhang, Fang Ding & Shuangxia Jin. The chromosome-scale reference genome of mirid bugs (Adelphocoris suturalis) genome provides insights into omnivory, insecticide resistance, and survival adaptation. BMC Biology 21, 195 (2023).