Cotton Assembly and Gene Annotation

August 1, 2023 | Genome

About Adelphocoris suturalis

Assembly

Annotation

Statistics

References

More information

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Adelphocoris suturalis Assembly and Gene Annotation

August 1, 2023 | Genome

About Adelphocoris suturalis

Adelphocoris suturalis (Hemiptera: Miridae) is a notorious agricultural pest, which causes serious economic losses to a diverse range of agricultural crops around the world. The poor understanding of its genomic characteristics has seriously hindered the establishment of sustainable and environment-friendly agricultural pest management through biotechnology and biological insecticides.

Assembly

Here, we report a chromosome-level assembled genome of A. suturalis by integrating Illumina short reads, PacBio, 10x Chromium, and Hi-C mapping technologies. The 1.29 Gb assembly contains twelve chromosomal pseudomolecules with a contig and scaffolds N50 of 2.8 Mb and 120.6 Mb, respectively.

Annotation

A comprehensive gene model prediction integrated with homology-based prediction, RNA-sequencing-assisted prediction and ab initio prediction identified a total of 20,010 protein-coding genes with 86.4% complete BUSCO genes. Of these, more than 94.3% genes are functionally annotated following searches of NR, GO, KEGG, Swiss-Prot and TrEMBL databases.

Statistics

Statistics Value
Sequencing platform Illumina+PacBio RS Ⅱ+PacBio Sequel Ⅱ
Genome sequencing depth (x) 260
Genome sequencing depth HiC 106
Estimated genome size (Gb) 1.29
Number of scaffolds 98
Sequenced genome size (Gb) 1.29
Contig N50 (Mb) 1.43
Scaffold N50 (Mb) 120.63
Longest scaffold (Mb) 188.72
GC content (%) 38.38
Repetitive sequences (%) 72.76
Annotated protein-coding genes 20,010
BUSCO completeness of assembly (%) 95.3
BUSCO completeness of annotation (%) 86.4
Statistics Value

References

Qin, Y., Sun, M., Li, W., Xu, M., Shao, L., Liu, Y., Zhao, G., Liu, Z., Xu, Z., You, J., Ye, Z., Xu, J., Yang, X., Wang, M., Lindsey, K., Zhang, X. and Tu, L. Single-cell RNA-seq reveals fate determination control of an individual fiber cell initiation in cotton (Gossypium hirsutum). Plant Biotechnol Journal. (2022)

More information

Tools (JBrowse, blast, Gene Search, Gene Expression and Sequence Fetch) used to Interaction with genomic information about this species can be found in InsectDB.

Cotton Single Cell Assembly and Gene Annotation

August 1, 2023 | Genome

About Adelphocoris suturalis

Assembly

Annotation

Statistics

References

More information

Corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt culpa. omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam, eaque ipsa quae.

perspiciatis unde omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam, eaque ipsa quae ab illo inventore veritatis et quasi architecto beatae vitae dicta sunt explicabo ipsam voluptatem quia voluptas sit aspernatur aut odit aut fugit, sed quia consequuntur magni dolores eos qui ratione voluptatem.